Fig 2: AB23A targets CD11b/CD18 and improves polarization of TAMs, thereby affecting tumor apoptosis. (A and B) IHC detected the expressions of iNOS and Arg-1. (C) ELISA was used to detect the levels of related markers in tumor tissue. (D) Tunel assay detected apoptosis. (E) Statistical analysis diagram of apoptosis rate. ***p < 0.001 vs A549; #p < 0.05, ###p < 0.001 vs A549+shRNA-NC; ???p < 0.001 vs A549+shRNA-ITGB2.

Fig 3: ITGAM deficiency attenuates the development of experimental AAA, vascular structural injury, and the inflammatory response. A, Representative photographs of the infrarenal abdominal aortas in saline- or CaCl2-treated WT and ITGAM(-/-) male mice. The black dotted lines indicate the edge of abdominal aortas. B, Kaplan–Meier survival curves of WT (n=15) and ITGAM(-/-) mice (n=15). No significant difference was found regarding the survival rate. C, Incidence of AAA formation in WT and ITGAM(-/-) mice in response to CaCl2. D, Maximal abdominal aortic diameter of WT (n=15) and ITGAM(-/-) mice (n=13) 6 weeks after being exposed to CaCl2 and the sham-treated mice (n=15). The data represent the mean±SEM. **P<0.01, ***P<0.001. E, Representative H&E, EVG, and Masson trichrome staining of the abdominal aortic tissues from the sham-treated, WT, and ITGAM(-/-) mice. Scale bar=100 µm. F and G, Quantification of elastin fragmentation and collagen deposition in the experimental groups (n=6), *P<0.05, **P<0.01, ****P<0.0001. H through K, Serum CCL2, TNF-a, IL-6, and IL-1ß levels in the experimental groups (n=6 for each group) detected by ELISA, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. AAA indicates abdominal aortic aneurysm; EVG, Elastica Van Gieson; H&E, hematoxylin and eosin; ITGAM, integrin subunit alpha M; and WT, wild-type.

Fig 4: Verification of the immunotype in mouse and patient samples by IHC staining. (a, c) Representative IHC images of CD206+ M2 macrophages (a) and FoxP3+ Tregs (c) in mouse PDAC samples (400×). (b, d) Statistics of the number of M2 macrophages (b) and Tregs (d) in a 400× field, each dot represents the mean of three random field in one sample. (e) Representative IHC images of CD3, CD11b, CD11c, CD19, CD163 and FoxP3 staining using human PDAC sample (400×, n = 8 in each group). (f) Statistics of the number of T cells, myeloid cells, DCs, B cells, M2 macrophages and Tregs in a 400× field, each dot represents the mean of three random field in one sample. n = 8 in each group. Donor pancreas: pancreas from organ donors, RPC: resectable pancreatic cancer, MPC: metastatic pancreatic cancer. For (b), (d) and (f), bar indicates mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, one-way ANOVA, multiple comparisons.

Fig 5: Has_ circ_ 0001326 regulates macrophages polarization via has-miR-136-5p/USP4 axis in THP-1 induced macrophages. (a) qRT-PCR was used to detect the expression of hsa-miR-136-5p, USP4, CD11B, INOS, ARG1, and FIZZ1 in CON (cotransfected si-NC and inhibitor NC), si-circ (cotransfected si-circ-3 and inhibitor NC), and si-circ+hsa-miR-136-5p inhibitor group. (b) Western blot assay was used to determine the expression of USP4, CD11B, INOS, ARG1, and FIZZ1 in CON, si-circ, and si-circ+hsa-miR-136-5p inhibitor group. (c) ELISA was used to evaluate the secretion of TNF-a, IL-6, IL-10 and IL-RA in CON, si-circ, and si-circ+hsa-miR-136-5p inhibitor group. (d) Dual luciferase reporter assay was used to further confirm the relationship between hsa_ circ_ 0001326 and hsa-miR-136-5p, the relationship between hsa-miR-136-5p and USP4; * indicates the p value less than 0.05.